Eppendorf 5331 MasterCycler Gradient Thermocycler


The Eppendorf 5331 Gradient MasterCycler is ideal for quantitative PCR applications, meeting all of the latest requirements with its outstanding speed and flexibility. The MasterCycler offers extremely fast temperature ramping, short detection times, and intuitive assay programming, which saves time to allow multiple accurate and reliable tests to be run per day.

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The Eppendorf 5331 simplifies PCR temperature testing, greatly speeding up the process by performing tests across a gradient temperature. The gradient function of this thermal cycler allows for a gradient difference of 1° - 20°C across the block, so multiple samples at different temperatures can be completed in just one run. The MasterCycler also offers the Peltier controlled block for precise homogeneity, guaranteeing the best reproducible results.


Seller refurbished. Excellent functional condition.

NOTE: Some of the units may have an aftermarket replacement lid latch knob installed to replace the original knob which is prone to cracking. The replacement knob may look different, but is stronger, and is fully functional.


  • Eppendorf 5331 MasterCycler Gradient Thermocycler
  • Power cord


Sample Capacity

96 x 0.2ml PCR tubes or 77 x 0.5ml PCR tubes or one 8 x 12 microtiter plate

Temperature Range

4°C - 99°C

Temperature Distribution

95°C ± 1.0°C

Temperature Accuracy

± 0.2°C

Pre-set Programs

100 built-in and approx 10 slots available for personal card

Maximum Cycles